Coding

Part:BBa_K3880008

Designed by: Honglei Jin   Group: iGEM21_GDSYZX   (2021-09-30)


CDO1(a cysteine dioxygenase)

CDO1 encodes a cysteine dioxygenase with 190 amino acids which has been found in Streptomyces sp. SCUT-3. CDO1 could catalyze cysteine’s oxidation to produce sulfite, which is the key factor for feather degradation. The previous study have demonstrated that overexpression of cdo1 increased bacterial single-cell sulfite production and feather protein conversion efficiency obviously. Co-overexpression of CDO1 and Sep39 under constitutive promoter ermE increased SCUT-3’s disulfide bond reduction and keratin hydrolysis to achieve higher feather degradation efficiency which exhibited highest total peptide and amino acid yields among SCUT-3, SCUT-Osep39 and SCUT-Ocdo1. However, the co-overexpression of CDO1 and Sep39 under this constitutive promoter ermE yielding fewer cells than SCUT-3 and Sep39 overexpression strain (SCUT-Osep39). To further improve the degradation efficiency without influencing the growth rate, we attempt to modified SCUT-3 through replacing the constitutive promoter with well-characterized promoters.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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